Immuno-engineered mitochondria for efficient therapy of acute organ injuries via modulation of inflammation and cell repair

Acute organ injuries are a major public health concern, driven by inflammation and mitochondrial dysfunction, leading to cell damage and organ failure. In this study, we engineered neutrophil membrane-fused mitochondria (nMITO), which combine the injury-targeting and anti-inflammatory properties of neutrophil membrane proteins with the cell-repairing function of mitochondria. nMITO effectively blocked inflammatory cascades and restored mitochondrial function, targeting both key mechanisms in acute organ injuries. Additionally, nMITO selectively targeted damaged endothelial cells via β-integrins and was delivered to injured tissues through tunneling nanotubes, enhancing their regulatory effects on inflammation and cell damage. In mouse models of acute myocardial injury, liver injury, and pancreatitis, nMITO significantly reduced immune dysfunction and repaired tissue damage. These findings suggest that nMITO is a promising therapeutic strategy for managing acute organ injuries., , , # Immuno-engineered mitochondria for efficient therapy of acute organ injuries via modulation of inflammation and cell repair

[https://doi.org/10.5061/dryad.rn8pk0pn4](https://doi.org/10.5061/dryad.rn8pk0pn4)

This dataset comprises the processed data tables corresponding to the main experimental figures and supplementary materials presented in the Science Advances article “Immuno-Engineered Mitochondria for Efficient Therapy of Acute Organ Injuries via Modulation of Inflammation and Cell Repair.” The data include quantitative measurements, imaging analyses, and in vivo/in vitro experimental results that evaluate mitochondrial engineering, inflammation modulation, and cell repair processes.

## Description of the data and file structure

The **data.zip** archive contains the following Excel files, each representing processed data for specific figures in the publication:

### **1.    Figure 1 Preparation and Characterization.xlsx**

**Preparation and Characterization of Neutrophil Membr...,

急性器官损伤是重大公共卫生问题，其诱因包括炎症反应与线粒体功能障碍，最终导致细胞损伤及器官衰竭。本研究中，我们构建了中性粒细胞膜融合线粒体（neutrophil membrane-fused mitochondria, nMITO），该结构整合了中性粒细胞膜蛋白的损伤靶向与抗炎特性，以及线粒体的细胞修复功能。nMITO可有效阻断炎症级联反应并恢复线粒体功能，同时作用于急性器官损伤的两大核心机制。此外，nMITO通过β整合素（β-integrins）选择性靶向受损内皮细胞，并经由隧道纳米管（tunneling nanotubes）递送至损伤组织，从而增强其对炎症及细胞损伤的调控效应。在急性心肌损伤、肝损伤及胰腺炎的小鼠模型中，nMITO显著改善免疫功能紊乱并修复组织损伤。这些发现表明，nMITO是应对急性器官损伤的极具潜力的治疗策略。,,, # 免疫工程化线粒体通过调控炎症与细胞修复实现急性器官损伤的高效治疗

[https://doi.org/10.5061/dryad.rn8pk0pn4]

本数据集包含与《Science Advances》文章《Immuno-Engineered Mitochondria for Efficient Therapy of Acute Organ Injuries via Modulation of Inflammation and Cell Repair》中主要实验图表及补充材料对应的处理后数据表。数据内容涵盖定量测量、成像分析以及评估线粒体工程化、炎症调控与细胞修复过程的体内/体外实验结果。

数据与文件结构描述

**data.zip**压缩包包含以下Excel文件，每个文件对应出版物中特定图表的处理后数据：
**1. 图1 制备与表征.xlsx**