Transcriptomic profiles of Candida albicans grown in the presence or absence of mucin glycans

We use high-throughput sequencing to profile the response of the opportunistic fungal pathogen Candida albicans to mucins and mucin-glycans from the mucosal niche. We find that C. albicans undergoes a genome-wide phenotypic shift in response to mucins and their attached glycans suppressing virulence-associated pathways. Overall design: Examination of differential gene expression in C. albicans in response to mucin sugars

我们采用高通量测序（high-throughput sequencing）技术，对机会致病性真菌病原体白色念珠菌（Candida albicans）响应黏膜微环境中的黏蛋白（mucins）及其结合型黏蛋白聚糖（mucin-glycans）的应答反应进行表征分析。研究发现，白色念珠菌在响应黏蛋白及其附着的聚糖时，会发生全基因组范围的表型重塑，该过程会抑制其毒力相关通路。整体实验设计：检测白色念珠菌在响应黏蛋白糖类时的差异基因表达情况。