Steady-state kinetic parameters for cleavage of Aβ1–40 and off-target peptides by wild-type NEP and NEPv.

*the best fit values of kcat and KM were determined by non-linear regression of activity data using the Michaelis-Menten equation. Values are quoted ± the average standard error generated from non-linear regression of data from two independent experiments.#the fold-change in kcat/KM is the ratio of the wild-type NEP kcat/KM to that of NEPv.

*通过米氏方程（Michaelis-Menten equation）对酶活性数据进行非线性回归分析，求得kcat与KM的最优拟合值；所有报道数值均以两次独立实验数据经非线性回归得到的平均标准误±形式标注。
#kcat/KM的倍数变化为野生型NEP的kcat/KM与NEPv的kcat/KM的比值。