Unraveling the role of human TSC-specific TFs [ChIP-Seq]

Orchestrated actions of tissue-specific enhancers and transcription factors (TFs) govern the development of the placenta which is an essential organ to support the growth of the fetus during pregnancy. However, human trophoblast stem cells (TSCs)-specific enhancers, TFs, and the mechanisms by which TFs modulate placental development are poorly understood. Here we investigated enhancers, super-enhancers (SE), SE-associated genes in human TSC using RNA-seq and ChIP-seq to interrogate the roles of SE-TFs in human TSCs. Overall design: ChIP-seq were performed for 6 transcription factors (EP300, FOS, GATA2, MAFK, TEAD4, and TFAP2C) in human trophoblast stem (TS) cells. Input was sequenced for a control.

组织特异性增强子与转录因子（Transcription Factors，TFs）的有序协同调控，主导了胎盘的发育进程。胎盘是妊娠期支持胎儿生长发育的必需器官。然而，目前针对人类滋养层干细胞（Trophoblast Stem Cells，TSCs）特异性的增强子、转录因子，以及转录因子调控胎盘发育的分子机制，相关研究认知仍较为匮乏。本研究采用RNA测序（RNA-seq）与染色质免疫共沉淀测序（ChIP-seq）技术，对人类滋养层干细胞中的增强子、超级增强子（Super-enhancers，SE）及其关联基因进行了系统性分析，以探究超级增强子相关转录因子在人类滋养层干细胞中的功能与作用。整体实验设计：本研究在人类滋养层干细胞（TS细胞）中，针对6种转录因子（EP300、FOS、GATA2、MAFK、TEAD4及TFAP2C）开展了染色质免疫共沉淀测序实验，并设置Input样本作为测序对照。