Identification of transcription in renal cortex from contrast-induced acute kidney injury mice.. Mus musculus

Twenty-four hours after establishment of the mouse CI-AKI model, kidneys were collected after isoflurane (2%) inhalation and cervical dislocation. Renal cortex was dissected quickly and preserved in liquid nitrogen. The process of sampling was very quick and all samples were kept at low temperature. After RNA isolation and purification, RNA quality was examined.

在构建小鼠造影剂诱导急性肾损伤（Contrast-Induced Acute Kidney Injury, CI-AKI）模型24小时后，采用2%异氟烷（isoflurane）吸入麻醉联合颈椎脱位法处死小鼠，摘取肾脏。迅速分离肾皮质并置于液氮中保存。采样过程全程快速完成，所有样本均维持低温状态。完成RNA分离与纯化后，对RNA质量进行检测。