Chronic exposure fish weights and plasma parameters

To fully assess the impacts of Deepwater Horizon (DWH) oil on the vertebrate stress response and the trajectory for recovery, our research integrated molecular, cellular, gland/organ and whole animal physiological and behavioral studies using a benthic marine teleost, the Gulf toadfish, a resident of the Gulf of Mexico. This study evaluated multiple pathways (e.g., pituitary fatigue, reduced receptor sensitivity, disruption or overstimulation of cortisol biosynthesis) and the consequence of these changes at the whole animal level (e.g., changes in carbohydrate metabolism, immune capacity and responses to natural stresses such as predation and social interaction). In this series of experiments, fish weight and plasma parameters such as cortisol concentrations were evaluated during long-term exposures to DWH oil. Toadfish were exposed to 0.006 (control), 0.692 (low), 1.432 (medium) and 3.525 (high) ug/L sum 50 total polycyclic aromatic hydrocarbon (PAH). Each exposure was followed by a complementary recovery period (i.e., no oil or PAH exposure, 7-28 days) to assess the recovery trajectory. These experiments were completed at Rosenstiel School of Marine and Atmospheric Science (RSMAS), University of Miami, Marine Technology & Life Sciences Seawater Complex (MTLSS) Building, room 211 between January 2019 to December 2019.

为全面评估深水地平线（Deepwater Horizon，DWH）原油对脊椎动物应激反应及恢复轨迹的影响，本研究以墨西哥湾的底栖海洋硬骨鱼类——海湾蟾鱼为研究对象，整合了分子、细胞、腺体/器官及整体动物层面的生理与行为学研究。本研究评估了多种通路（如垂体疲劳、受体敏感性降低、皮质醇生物合成紊乱或过度刺激），以及这些变化在整体动物层面的后果（如碳水化合物代谢改变、免疫能力变化，以及对捕食、社交互动等自然应激的响应变化）。在这一系列实验中，研究人员评估了长期暴露于DWH原油期间鱼类的体重及皮质醇浓度等血浆参数。海湾蟾鱼被暴露于50种多环芳烃（PAH）总浓度分别为0.006（对照）、0.692（低）、1.432（中）和3.525（高）μg/L的环境中。每次暴露后均设置补充恢复期（即无原油或PAH暴露，持续7-28天），以评估其恢复轨迹。这些实验于2019年1月至12月期间，在迈阿密大学罗森斯蒂尔海洋与大气科学学院（RSMAS）的海洋技术与生命科学海水综合体（MTLSS）大楼211室完成。