Comparison of mouse and human retinal pigment epithelium gene expression profiles reveals interspecies similarities and differences. Mus musculus

To determine mouse retinal pigment epithelium (RPE) specific gene expression, we compared the gene expression profile of the RPE to the gene expression of its adjacent layers: the choroid (CH) and photoreceptors (PR). We collected the tissues using the meticulous laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. Compared RPE gene expression with CH gene expression, and with PR gene expression Overall design: We performed the microarrays against a common reference sample, namely mouse RPE/choroid RNA. We performed 3 replicates of every tissue: CH, RPE and PR.

为探究小鼠视网膜色素上皮（retinal pigment epithelium, RPE）的特异性基因表达模式，我们将该组织的基因表达谱与其相邻的两层组织——脉络膜（choroid, CH）和光感受器（photoreceptors, PR）的基因表达谱进行了对照分析。我们采用精密激光显微切割技术采集各组织样本，随后依次开展RNA分离、扩增、标记，并将标记产物与44k安捷伦（Agilent）微阵列进行杂交。我们分别将RPE的基因表达谱与脉络膜（CH）、光感受器（PR）的基因表达谱进行了比较。总体实验设计：本研究以小鼠RPE/脉络膜RNA作为通用参考样本进行微阵列实验，所有组织样本（CH、RPE、PR）均设置3次生物学重复。