RNAscope Imaging of Enteric Glial Cells

These images were taken to test the hypothesis that glial cells within the myenteric ganglia of the enteric nervous system are enriched for cells that transcribe Gfap, Sox2, Slc18a2, Ramp1, and Cpe in adult mice as well as in young mice prior to weaning. RNAscope imaging of was performed on whole-mount longitudinal muscle-myenteric plexus (LMMP) tissue from the small intestine of Plp1::GFP mice. We found that glial cells within ganglia are markedly enriched for transcripts of these genes in both young mice (age P14) and adult mice (age 10.5 weeks).

本数据集所收录的成像图像，均用于验证如下假说：成年小鼠及断奶前幼鼠的肠神经系统肌间神经节内的神经胶质细胞中，转录Gfap、Sox2、Slc18a2、Ramp1及Cpe基因的细胞呈现富集状态。实验对Plp1::GFP转基因小鼠小肠的全铺制纵行肌-肌间神经丛（longitudinal muscle-myenteric plexus, LMMP）组织开展了RNAscope成像检测。研究结果表明，在幼鼠（出生后14日龄，P14）与成年小鼠（10.5周龄）的肌间神经节内，神经胶质细胞均显著富集上述基因的转录本。