Temporal Quantitative Proteomic Profiling of SH-SY5Y and IMR-32 Neuroblastoma Cells During All-trans Retinoic Acid-Induced Neuronal Differentiation

The human neuroblastoma cell lines SH-SY5Y and IMR-32 can be differentiated into neuron-like phenotypes through treatment with all-trans retinoic acid (ATRA). After differentiation, these cell lines are extensively utilized as in vitro models to study various aspects of neuronal cell biology. However, temporal and quantitative profiling of the proteome and phosphoproteome of SH-SY5Y and IMR-32 cells throughout ATRA-induced differentiation has been limited. Here, we performed relative quantification of the proteomes of SH-SY5Y and IMR-32 cells at multiple time points during ATRA-induced differentiation. The data presented serve as a valuable resource for investigating temporal protein and phosphoprotein abundance changes in SH-SY5Y and IMR-32 cells during ATRA-induced differentiation.

人神经母细胞瘤细胞系SH-SY5Y与IMR-32可通过全反式维甲酸（all-trans retinoic acid, ATRA）处理诱导分化为神经元样表型。分化完成后，该类细胞系被广泛用作体外模型，用于神经元细胞生物学诸多方面的研究。然而，针对全反式维甲酸诱导分化全过程中SH-SY5Y与IMR-32细胞的蛋白质组与磷酸化蛋白质组的时序定量谱分析研究仍较为有限。本研究对全反式维甲酸诱导分化过程中多个时间节点下的SH-SY5Y与IMR-32细胞的蛋白质组进行了相对定量分析。本研究呈现的数据集，可为探索全反式维甲酸诱导分化过程中SH-SY5Y与IMR-32细胞内蛋白质与磷酸化蛋白质丰度的时序变化提供宝贵的研究资源。