Transcriptomic expression profiles of SPF chicken-spleen infected with duck-origin H7N9 subtype avian influenza virus and fowl adenovirus-4

With the purpose to elucidate the expression changes of host genes of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus at 24 hours post-infection(hpi) and fowl adenovirus-4 at 48 dpi. The spleens of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus and fowl adenovirus-4 were collected and high throughout sequenced. Compared with the control group, there were 2426 differentially expressed genes were obtained in the duck-origin H7N9 subtype avian influenza virus group, including 913 up-regulated genes and 1513 down-regulated genes, and there were 1534 differentially expressed genes were obtained in the fowl adenovirus-4 group, including 632 up-regulated genes and 902 down-regulated genes. Overall design: Spleen mRNA profiles of SPF chickens infected with duck-origin H7N9 AIV at 24 hpi and with Fowl adenovirus-4 at 48 hpi and control were generated by deep sequencing, in triplicate, using Illumina X ten.

本研究旨在解析感染鸭源H7N9亚型禽流感病毒（duck-origin H7N9 subtype avian influenza virus）的无特定病原体（Specific Pathogen Free, SPF）鸡，于感染后24小时（hpi）以及感染禽腺病毒4型（fowl adenovirus-4）后48 hpi时的宿主基因表达变化。收集感染鸭源H7N9亚型禽流感病毒与禽腺病毒4型的SPF鸡脾脏组织，开展高通量测序。与对照组相比，鸭源H7N9亚型禽流感病毒感染组共筛选得到2426个差异表达基因，其中上调基因913个、下调基因1513个；禽腺病毒4型感染组共筛选得到1534个差异表达基因，其中上调基因632个、下调基因902个。实验设计：本研究采用Illumina X ten测序平台对三组样本进行深度测序，分别为感染鸭源H7N9亚型禽流感病毒（Avian Influenza Virus, AIV）后24 hpi的SPF鸡脾脏、感染禽腺病毒4型后48 hpi的SPF鸡脾脏，以及对照组SPF鸡脾脏，每组设置3次生物学重复。