Growth rate of Paracentrotus lividus larvae under different temperature, pH and lithium conditions

A four-day experiment was conducted in October 2022 to resolve performance curves for temperature, pH and lithium contamination and understand their combined effects on Paracentrotus lividus larval growth. Adults were collected at Cap d'Ail, France and transferred to the Marine Environment Laboratories of the International Atomic Energy Agency (IAEA) in Monaco, where fertilization and the subsequent aquarium experiments were carried out in closed borosilicate bottles. Larval body length was measured after the experiment from microscopic photographs of the larvae, and growth rate calculated by dividing the body length with days since fertilization. At the end of the experiment, lithium concentration was measured with optical emission spectrometry, pH on the total scale with a TRIS-calibrated electrode, total alkalinity with open cell potentiometric titration, salinity with a salinometer, and the carbonate system calculated using the seacarb package in R. Temperature was measured continuously and averaged. This dataset provides the above parameters.

本研究于2022年10月开展了一项为期四天的实验，旨在厘清温度、pH值与锂污染的效应曲线，并探明三者对地中海紫海胆（Paracentrotus lividus）幼体生长的联合影响。实验所用成体海胆采集自法国卡普戴尔（Cap d'Ail），随后被转运至位于摩纳哥的国际原子能机构（International Atomic Energy Agency, IAEA）海洋环境实验室，实验中的受精过程及后续的水族箱培养均在密闭硼硅酸盐玻璃瓶中完成。实验结束后，研究人员通过幼体的显微照片测量其体长，并通过将体长除以受精后的天数计算得到生长速率。实验收尾阶段，研究人员采用光学发射光谱法测定锂浓度，以三羟甲基氨基甲烷（TRIS）校准电极测定总尺度pH值，采用开放式电池电位滴定法测定总碱度，通过盐度计测量盐度，并利用R语言中的seacarb程序包计算碳酸盐体系参数。温度参数采用连续监测后取平均值的方式获取。本数据集包含上述所有测定参数。