ExtFig9C_TCID50_M-PR8-WT-dX-8h_R1_LG389.HEIC

MDCK cells were infected at MOI 0.05 with the indicated viruses. Supernatants were collected at 1, 8, 24 and 48 hours post infection and viral titers were quantified by TCID50. Images show the TCID50 plates with scoring.

以感染复数（MOI, multiplicity of infection）为0.05的剂量，使用指定病毒感染MDCK细胞（Madin-Darby Canine Kidney cells）。分别于感染后1、8、24及48小时收集细胞培养上清液，通过50%组织细胞感染量（TCID₅₀, 50% Tissue Culture Infectious Dose）实验对病毒滴度进行定量检测。所附图像展示了完成计分的TCID₅₀检测板。