Table S1. List of the up-regulated proteins found in the secretome of IL-1β primed MSC.

In the present study, we found that, IL-1β-primed gingival MSCs (IL-MSCs) promoted cell migration, dermal-epidermal junction formation and inflammation reduction in vitro, as well as improved epidermal substitute engraftment in vivo. IL-MSCs had different secretory profiles from naive gingival MSCs (NV-MSCs), characterized by an overexpression of TGF-β and MMP pathway agonists. Eventually, MMP-1, MMP-9 and TGF-β1 appeared to be critically involved in IL-MSC mechanisms of action.

本研究结果显示，经白细胞介素1β (IL-1β) 预处理的牙龈间充质干细胞 (gingival MSCs, IL-MSCs) 可在体外促进细胞迁移、真皮表皮连接 (dermal-epidermal junction) 形成与炎症缓解，同时在体内可提升表皮替代物的移植成活率。与未经预处理的牙龈间充质干细胞 (NV-MSCs) 相比，IL-MSCs具有独特的分泌谱 (secretory profiles) 特征，表现为转化生长因子β (TGF-β) 与基质金属蛋白酶 (MMP) 通路激动剂的高表达。最终研究表明，基质金属蛋白酶1 (MMP-1)、基质金属蛋白酶9 (MMP-9) 及转化生长因子β1 (TGF-β1) 在IL-MSCs的作用机制中发挥关键调控作用。