Regional heterogeneity of glioblastoma upon single-cell RNA sequencing

Using spontaneous mouse GBM model, regional dissection of cortical margin, callosal margin, and tumor center was done for single-cell RNA sequencing to investigate molecular characteristics of marginal cancer cells. Cancer cells were sorted from cortex, corpus callosum, and tumor center respectively, then single-cell RNA libraries were made independently.

本研究采用自发性小鼠胶质母细胞瘤（Glioblastoma, GBM）模型，对皮质边缘、胼胝体边缘及肿瘤中心进行区域解剖，开展单细胞RNA测序以探究边缘癌细胞的分子特征。研究人员分别从大脑皮质、胼胝体及肿瘤中心中分选癌细胞，随后独立构建单细胞RNA测序文库。