Expression profiling of mammalian Schwann cells in response to treatment with NRG and/or IGF

Comparison of the changes in mitochondrial gene expression of cells in which extracellular growth factors and/or mitogens have been added. Keywords: Input of growth and mitogenic pathways in mitochondria biogenesis Primary Rat Schwann cells were arrested and synchronized with aphidicolin for 24 hours in 3% FCS containing medium. Cells where then transferred to defined conditions (no factors) with aphidocolin for another 24 hours before appropriate factors were applied for 15 hours prior to cell collection.

本研究对比了添加细胞外生长因子和/或有丝分裂原后细胞线粒体基因表达的变化。关键词：线粒体生物发生中生长通路与有丝分裂原通路的输入调控。实验流程：将原代大鼠雪旺细胞（Primary Rat Schwann cells）置于含3%胎牛血清（FCS）的培养基中，使用阿非迪霉素（aphidicolin）进行24小时的细胞周期阻滞与同步化处理；随后将细胞转移至无外源添加因子的限定培养体系，继续以阿非迪霉素处理24小时；之后加入适宜的生长因子处理15小时，最终收集细胞。