RNA transcriptome sequencing analysis of Control HASMCs, RSL3-induced HASMCs, and RSL3+Silibinin HASMCs

The mechanism of aortic dissection (AD) remains unclear. Several studies have demonstrated the potential protective and therapeutic applications of silibinin in cardiovascular diseases (CVDs), particularly in terms of its antioxidant, hypolipidemic, hypoglycemic, anti-hypertensive, and cardioprotective effects. The precise mechanism of how SIL impacts the occurrence and development of AD is still not completely understood. RNA transcriptome sequencing analysis was performed in control HASMCs, RSL3-induced HASMCs, and RSL3+SIL HASMCs. The HASMCs were treated with RSL3 (50 nM) for 12 h to create the ferroptosis cell model. In the RSL3+SIL HASMCs group, 10 uM SIL was used.

主动脉夹层（aortic dissection, AD）的发病机制仍未明确。多项研究已证实水飞蓟宾（silibinin）在心血管疾病（cardiovascular diseases, CVDs）中具有潜在的保护与治疗应用价值，其抗氧化、调脂、降糖、降压及心脏保护作用尤为显著。但SIL影响主动脉夹层发生与发展的确切机制仍未完全阐明。本研究对对照组人主动脉平滑肌细胞（human aortic smooth muscle cells, HASMCs）、RSL3诱导的人主动脉平滑肌细胞以及RSL3联合SIL处理的人主动脉平滑肌细胞开展了RNA转录组测序分析。研究人员以50 nM RSL3处理细胞12小时，以构建铁死亡细胞模型；在RSL3联合SIL处理组中，采用10 μM SIL进行干预。