<b>Supplemental material</b>

This study identified that USP35 regulates proliferation and glycolysis of breast cancer cells by mediating the ubiquitination level of PFK-1. The USP35/PFK-1 axis offers novel insight for the treatment of breast cancer.本研究发现，USP35通过介导PFK-1的泛素化水平调控乳腺癌细胞的增殖与糖酵解过程。USP35/PFK-1分子轴为乳腺癌治疗提供了新的见解。