Gene expression profile (RNAseq) of peritoneal mouse macrophages in Endotoxic shock and Tolerance. Gene expression profile (RNAseq) of peritoneal mouse macrophages in Endotoxic shock and Tolerance

Purpose: Characterize the gene expression profile of of peritoneal mouse macrophages in Endotoxic shock and Tolerance through RNA sequencing Methods: RNA sequencing of RNA from peritoneal macrophages in Endotoxic shock and Tolerance isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) Results: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression. Conclusions: Endotoxic shock and Tolerance peritoneal mouse macrophages display differential gene expression. Overall design: RNA sequencing low Imput of RNA from peritoneal macrophages isolated by peritoneal lavage and FACS sorting (F4/80+ CD11b+) (2-3 mice pooled for each sample)

研究目的：通过RNA测序（RNA sequencing）表征内毒素休克与免疫耐受小鼠腹腔巨噬细胞的基因表达谱。
实验方法：通过腹腔灌洗分离小鼠腹腔巨噬细胞，经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）筛选标记为F4/80+ CD11b+的细胞，提取内毒素休克与免疫耐受组小鼠腹腔巨噬细胞的RNA并进行RNA测序。
实验结果：内毒素休克与免疫耐受小鼠腹腔巨噬细胞呈现差异化基因表达特征。
研究结论：内毒素休克与免疫耐受小鼠腹腔巨噬细胞存在差异化基因表达。
整体实验设计：对经腹腔灌洗及荧光激活细胞分选（F4/80+ CD11b+）分离得到的小鼠腹腔巨噬细胞的低起始量RNA进行测序，每个样本混合2~3只小鼠的细胞。