Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes

It is currently unknown whether all Plasmodium falciparum infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso. We quantified the number of sporozoites expelled into artificial skin in relation to intact oocysts, ruptured oocysts, and residual salivary gland sporozoites. Sporozoites were quantified by highly sensitive qPCR; intact and ruptured oocysts by fluorescence microscopy following antibody staining of circumsporozoite protein. In laboratory conditions, higher total sporozoite burden in mosquitoes was associated with a shorter duration of sporogony (p<0.001). Overall, 53% (116/216) of P. falciparum infected An. stephensi mosquitoes expelled sporozoites into artificial skin. The medians of expelled and residual salivary gland sporozoites were 136 (IQR: 34-501) and 23,947 (IQR: 9127-78,380), respectively. There was a strong positive correlation between rupture..., This work involves data collected with in vitro cultured gametocytes and gametocytes circulating in naturally infected individuals. Parasite development in mosquitoes was examined in relation to parasite burden in mosquitoes. Data collected centered around the expelling of sporozoites by mosquitoes infected with high and low infection burden. Parasites were quantified primarily by qPCR, supplemented by microscopy., , # \"Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes \"

[https://doi.org/10.5061/dryad.dbrv15f89](https://doi.org/10.5061/dryad.dbrv15f89)

Datasets are linked to individual display figures in the article.

## Description of files

#### File Name: Data\_for\_Figure\_1B\_oocyst\_density\_and\_prevalence.xlsx

**Description of file:**
Data that is generated in mosquito feeding assays with cultured gametocytes in Nijmegen The Netherlands. The set contains three conditions: standard, routine feedings for generating infectious mosquitoes; EIP, feedings performed to conduct EIP experiments in the paper aiming for with low and high infected mosquitoes; Skin, feedings performed to conduct Skin experiments in the paper aiming for low and high infected mosquitoes.

**Variables:**

* Condition: Standard, Skin, EIP
* Oocyst density: Mean of oocysts counted in at least 10 mosquitoes that derived from the sa...

目前尚不清楚所有感染恶性疟原虫（Plasmodium falciparum）的蚊子是否具有同等的感染能力。本研究分别在荷兰利用体外培养的疟原虫配子体，以及在布基纳法索利用当地自然流行的疟原虫毒株，评估了疟原虫在蚊体内的孢子增殖发育过程。我们定量测定了按蚊向人工皮肤内排出的子孢子数量，并分析其与完整卵囊、破裂卵囊及唾液腺残留子孢子的相关性。子孢子载量通过高灵敏度定量聚合酶链反应（qPCR）进行测定；通过对环子孢子蛋白进行抗体染色后辅以荧光显微镜观察，计数完整与破裂的卵囊。

实验室条件下，蚊子体内总子孢子负荷越高，孢子增殖周期越短（p<0.001）。总体而言，53%（116/216）的感染恶性疟原虫的斯氏按蚊（Anopheles stephensi）可向人工皮肤内排出子孢子。排出的子孢子与唾液腺残留子孢子的中位数分别为136（IQR: 34~501）与23947（IQR: 9127~78380）。卵囊破裂情况与……之间存在显著正相关。

本研究的数据来源于两部分：一是体外培养的疟原虫配子体实验，二是自然感染个体体内循环的疟原虫配子体实验。我们分析了疟原虫在蚊体内的发育情况与蚊子寄生虫负荷之间的关联，所收集的数据核心围绕不同感染负荷的蚊子排出子孢子的情况展开。寄生虫载量的定量主要通过qPCR完成，并辅以显微镜镜检。

# "感染实验室培养与自然流行恶性疟原虫配子体的按蚊子孢子排出量定量研究"
[https://doi.org/10.5061/dryad.dbrv15f89](https://doi.org/10.5061/dryad.dbrv15f89)

数据集与文章中的各展示图一一对应。

## 文件描述
#### 文件名：Data_for_Figure_1B_oocyst_density_and_prevalence.xlsx
**文件说明：**
本数据来自荷兰奈梅亨地区开展的、使用体外培养配子体的蚊子喂血实验。数据集包含三种实验条件：①标准组（Standard）：用于制备感染性蚊子的常规喂血操作；②EIP组：为开展本研究中EIP实验设置的喂血操作，旨在获得低、高感染负荷的蚊子；③Skin组（Skin）：为开展本研究中皮肤实验设置的喂血操作，旨在获得低、高感染负荷的蚊子。

**变量说明：**
* 实验条件（Condition）：Standard（标准组）、Skin（皮肤实验组）、EIP（EIP组）
* 卵囊密度（Oocyst density）：对至少10只来源于……的蚊子所计数的卵囊数的平均值