Atomic force microscopy indentation data of stiff and compliant polyacrylamide hydrogels

<b><br>Atomic force microscopy indentation data of stiff and compliant polyacrylamide hydrogels</b><br><br>This dataset consists of 110 force-distance curves of two (stiff and compliant) polyacrylamide (PAAm) gels recorded using atomic force microscopy. Each file represents one measurement. The file names indicate whether a measurement was performed on the stiff or on the compliant gel. For each gel, curves were recorded from six regions of interest (ROIs).<br><br>The data were recorded using a JPK NanoWizard 4 and a PNP-TR-TL cantilever (Nanoworld) with a spring constant of 0.08 N/m. For probing, a bead with a radius of 5 µm was attached to the cantilever. The cantilever was calibrated using the thermal noise method in phosphate buffered saline (PBS) at room temperature.<br><br>The hydrogels were produced according to the protocol described in [1]. The gels have an expected Young's modulus of 0.8 to 1.2 kPa (compliant) and 25 to 28 kPa (stiff).<br><br><br><b>References</b><br>[1] G. Rosso et al., Schwann cells and neurite outgrowth from embryonic dorsal root ganglions are highly mechanosensitive, Nanomedicine: Nanotechnology, Biology and Medicine, 2016, doi:10.1016/j.nano.2016.06.011<br><br>

<b><br>刚性与柔性聚丙烯酰胺(polyacrylamide, PAAm)水凝胶的原子力显微镜(Atomic force microscopy, AFM)压痕数据</b><br><br>本数据集包含通过原子力显微镜采集的两种（刚性与柔性）聚丙烯酰胺凝胶的110条力-距离曲线。每个文件对应一次测量，文件名标注了该测量是在刚性凝胶还是柔性凝胶上完成的。针对每种凝胶，均从6个感兴趣区域(Region of Interest, ROI)采集了曲线。<br><br>数据采集使用JPK NanoWizard 4型原子力显微镜以及PNP-TR-TL型悬臂梁（Nanoworld公司），其弹簧常数为0.08 N/m。测量过程中，半径为5 µm的微球被附着于悬臂梁末端。悬臂梁的校准采用热噪声法，校准环境为室温下的磷酸盐缓冲液(Phosphate Buffered Saline, PBS)。<br><br>该水凝胶的制备参照文献[1]所述方案。两种凝胶的预期杨氏模量分别为0.8~1.2 kPa（柔性组）与25~28 kPa（刚性组）。<br><br><b>参考文献</b><br>[1] G. Rosso等，《雪旺细胞与胚胎背根神经节的神经突生长具有高度机械敏感性》，《纳米医学：纳米技术、生物学与医学》，2016年，doi:10.1016/j.nano.2016.06.011