Inline holographic microscopy through a fibre imaging bundle. Fig. 4.

This dataset and code demonstrates the change in resolution with depth in a fibre bundle holographic microcsope with partially coherent illumination. The sample is 5 micron diameter microspheres on a 1 mm thick glass slide. The slide was back illuminated by 450 nm LED light coupled through a multimode fibre with a 50 micron diameter core. The illumination fiber was approximately 15 mm behind the slide. The hologram was collected by a Fujikura FIGH-30-650S fibre bundle moved between 0 and 3 mm in front of the microspheres, in steps of 100 microns. At the proximal end, this was imaged onto a CMOS camera via a 20X objective and a tube lens, giving an effective pixel size of 0.44 microns. The file <i>recon_microspheres_dist </i>is used to create the images from the raw holograms, and the file <i>analyse_microspheres_dist</i> is used to measure the change in apparent size of the spheres as they are moved away from the bundle.

本数据集与配套代码展示了部分相干照明下纤维束全息显微镜的分辨率随成像深度的变化规律。实验样本为置于1mm厚载玻片上的直径5微米微球。载玻片采用450nm LED光进行背照照明，该光线通过纤芯直径50微米的多模光纤耦合注入。照明光纤位于载玻片后方约15mm处。全息图由藤仓（Fujikura）FIGH-30-650S型纤维束采集，该纤维束在微球前方0至3mm范围内以100微米为步长进行移动扫描。在光纤束的近端，采集信号通过20倍物镜与管透镜成像至CMOS相机，最终获得0.44微米的有效像素尺寸。脚本文件`recon_microspheres_dist`用于从原始全息图中重建图像，而`analyse_microspheres_dist`则用于测量微球在远离纤维束过程中表观尺寸的变化。