Possible mRNA targets of ZC3H11.

The Table includes all mRNAs that were at least 3-fold enriched in the ZC3H11 bound fraction, with more than 40 reads in the bound fraction and more than10 reads per million in the unbound fraction. The full lists are in Supplementary Table S1. The genes are grouped according to predicted protein function. All RNASeq results were normalised to reads per million per kilobase (rpkm) before the ratios were calculated. PC bind: Ratio of eluate to flow-through in the binding experiment. “Description” is the functional classification in TriTrypDB, with some additions and modifications. PC HS Seq: RNA rpm for procyclics heated to 41°C for 1 h, relative to procyclics kept at 27°C. PC HS RNAi: RNA read counts per million in procyclics with ZC3H11 RNAi, heated to 41°C for 1 h, divided by the read counts in wild-type procyclics heated to 41°C;
*Northern blot result between 1× and 1.5× (Supplementary Figure S5);
§Northern or published microarray result 5–6× [17]. BS RNAi Seq: effect of RNAi targeting ZC3H11 in bloodstream forms, relative to wild-type. “(AUU)3”: “yes” indicates the presence of (at least) the 12mer AUUAUUAUUAUU in the 3′-UTR, (yes) is a perfectly-matching 11mer. PPIase = peptidyl prolyl cis-trans isomerase; TM = trans-membrane domain, DUF866 = domain of unknown function. For multi-copy genes only one member is shown. They are HSP83 (Tb917.10.10890-10980), HSP60 (Tb927.10.6400 and 6510), and HSP70.

本表格收录了所有在ZC3H11结合组分中富集倍数至少达3倍的mRNA，且结合组分的序列读取数（reads）需超过40，未结合组分中的每百万序列读取数需超过10。完整列表见补充表S1。基因按照预测的蛋白质功能进行分组。所有RNA测序（RNASeq）结果在计算比值前，均已按每千碱基每百万序列读取数（rpkm，reads per million per kilobase）进行标准化处理。PC结合比（PC bind）：结合实验中洗脱组分与流过组分的比值。"Description"对应的功能描述为TriTrypDB数据库中的功能分类，并进行了部分补充与修改。PC热激测序（PC HS Seq）：经41℃热激1小时的前循环型锥虫的RNA每百万读取数（rpm），相对于维持在27℃的前循环型锥虫。PC热激RNA干扰测序（PC HS RNAi）：经41℃热激1小时的ZC3H11 RNA干扰（RNAi）前循环型锥虫的每百万RNA读取数，除以经41℃热激1小时的野生型前循环型锥虫的读取数；* 标注星号的结果为Northern blot实验结果，其信号倍数介于1×至1.5×之间，详见补充图S5；§ 标注段落符号的结果为Northern blot或已发表的微阵列（microarray）实验结果，其信号倍数为5–6×[17]。BS RNAi测序（BS RNAi Seq）：靶向ZC3H11的RNA干扰对血流型锥虫的影响效应，相对于野生型血流型锥虫。"(AUU)3"：标记为"yes"表示在3′非翻译区（3′-UTR）中存在至少1条12聚体序列AUUAUUAUUAUU，标记为(yes)表示存在完全匹配的11聚体序列。PPIase=肽基脯氨酰顺反异构酶（peptidyl prolyl cis-trans isomerase）；TM=跨膜结构域（trans-membrane domain）；DUF866=功能未知结构域（domain of unknown function）。多拷贝基因仅展示其中一个成员，分别为HSP83（Tb917.10.10890-10980）、HSP60（Tb927.10.6400与6510）以及HSP70。