Supporting data for Identification of a novel m6Am reader and characterization of its role in mRNA transcriptional regulation

This dataset supports the study titled <i>"</i><i>Identification of a novel m6Am reader and characterization of its role in mRNA transcriptional regulation</i><i>"</i> . The research investigates the epitranscriptomic modification m6Am and its role in transcriptional regulation through interaction with the cleavage and polyadenylation factor PCF11. By integrating multiple transcriptomic and biochemical approaches, the study reveals how m6Am modulates premature transcription termination and contributes to neuroblastoma cell differentiation.The data were generated from various high-throughput sequencing experiments and molecular assays conducted primarily in human neuroblastoma BE(2)C cells. The sample size varies by assay, with biological replicates included for key experiments to ensure reproducibility.The dataset includes:BED files for genomic visualization of peaks and features (viewable in IGV).<b>RNA-seq</b> and <b>SLAM-seq</b> data to quantify gene expression and transcriptional dynamics.<b>ChIP-seq</b> data to map PCF11 and RNA Polymerase II binding landscapes.<b>fPAR-CLIP</b> data to identify transcriptome-wide RNA-protein interaction sites.<b>m6ACE-seq</b> data to profile m6Am methylation sites.<b>PCT-seq</b> data to detect prematurely cleaved transcripts genome-wide.Related numerical data used to generate box-and-whisker plots have also been exported as tables for condition-wise comparison.Reporter assay data to validate the role of m6Am in transcription.Unprocessed images (Figures 2C, 6A, 6D–6F, S1N, S2A, S2C, S2I, S2J, S3A, S4D, S4G, S6A, and S6J, western blots. Figure S2B, non-denaturing PAGE. Figure S2C, denaturing PAGE. Figure S2K, Coomasie.) —have been uploaded to support the quantification of assay readouts under different treatments.All data files are processed and well-organized to facilitate reuse by other researchers. No personally identifiable or confidential information is included.

本数据集支持题为《新型m6Am阅读器的鉴定及其在mRNA转录调控中的作用表征》的研究。该研究聚焦于表观转录组修饰m6Am，并通过其与切割及多聚腺苷酸化因子PCF11的相互作用，探究其在转录调控中的功能。本研究整合多种转录组学与生物化学手段，揭示了m6Am如何调控转录提前终止，并参与神经母细胞瘤细胞的分化过程。相关数据主要通过在人神经母细胞瘤BE(2)C细胞中开展的多种高通量测序实验与分子实验获得。各实验的样本量存在差异，关键实验均设置生物学重复以确保结果可复现。本数据集包含以下内容：用于基因组峰与特征可视化的BED文件（可在IGV（Integrative Genomics Viewer）中查看）；RNA测序（RNA-seq）与SLAM-seq数据，用于定量基因表达水平与转录动态变化；染色质免疫共沉淀测序（ChIP-seq）数据，用于绘制PCF11与RNA聚合酶II的结合全景；fPAR-CLIP数据，用于在全转录组范围内鉴定RNA-蛋白质相互作用位点；m6ACE-seq数据，用于分析m6Am甲基化位点；PCT-seq数据，用于在全基因组范围内检测提前切割的转录本；用于生成箱线图的相关数值数据已导出为表格，用于不同处理条件下的组间比较；报告基因实验数据，用于验证m6Am在转录过程中的功能；未处理的原始图像（包括图2C、6A、6D–6F、S1N、S2A、S2C、S2I、S2J、S3A、S4D、S4G、S6A及S6J的免疫印迹实验结果；图S2B的非变性聚丙烯酰胺凝胶电泳结果；图S2C的变性聚丙烯酰胺凝胶电泳结果；图S2K的考马斯亮蓝染色结果）已上传，用于支持不同处理条件下实验读数的定量分析。所有数据文件均经过规范化处理与整理，便于其他研究人员复用。本数据集未包含任何个人可识别信息或机密内容。