RNA-seq of human epithelium-only colonic and small intestinal organoids derived from intestinal resections, biopsy specimens and cryopreserved crypts. RNA-seq of human epithelium-only colonic and small intestinal organoids derived from intestinal resections, biopsy specimens and cryopreserved crypts

Patient-derived gastrointestinal epithelium-only organoids from the small and large intestine, also referred to as colonoids and enteroids, were generated as part of the development of an on-going organoid biobank at the Michigan Medicine Translational Tissue Modeling Laboratory (TTML) (www.UmichTTML.org). Gene expression in organoids was characterized using RNA-sequencing

本研究所使用的类器官（organoids）为源自患者的小肠与大肠仅含胃肠道上皮的样本，此类类器官亦可分别称为结肠类器官（colonoids）与小肠类器官（enteroids）。该类器官的制备工作隶属于密歇根医学转化组织建模实验室（Michigan Medicine Translational Tissue Modeling Laboratory, TTML，www.UmichTTML.org）正在推进的类器官生物样本库构建项目。研究团队通过RNA测序（RNA-sequencing）对该类器官的基因表达特征进行了表征分析。