Cell Spheroid Model of Calcific Aortic Valve Disease - Apoptosis and Oxidative Stress

Valvular intersitital cells cultured as spheroids undergo apoptosis and subsequent calcification. Antioxidants reduces the apoptosis and calcification to varying extents suggesting that the most effective antioxidant, ascorbic acid, plays additional roles e.g., inducing ECM deposition.

以细胞球（spheroids）形式培养的瓣膜间质细胞（Valvular interstitial cells）会发生细胞凋亡，并随后出现钙化。抗氧化剂可在不同程度上减轻细胞凋亡与钙化，这提示效果最佳的抗氧化剂——抗坏血酸（ascorbic acid）还可发挥额外作用，例如诱导细胞外基质（Extracellular Matrix，ECM）沉积。