In vivo BioID Mouse heart masspec data

Cardiac excitation-contraction (E-C) coupling requires dyads, the nanoscopic microdomains formed adjacent to Z-lines by apposition of transverse (T)-tubules and junctional sarcoplasmic reticulum (jSR). Disruption of dyad architecture and function are common features of diseased cardiomyocytes. However, little is known about the mechanisms that modulate dyad organization during cardiac homeostasis and disease. Here, we used proximity proteomics in intact, living hearts to identify proteins enriched near dyads.

心脏兴奋-收缩（E-C）耦联依赖于二联体（dyads）——该结构是由横管（transverse (T)-tubules）与连接型肌浆网（junctional sarcoplasmic reticulum, jSR）相互贴合，在Z盘（Z-lines）附近形成的纳米级微区。二联体结构与功能的破坏是病变心肌细胞的典型特征。然而，目前对于心脏稳态与疾病进程中调控二联体组织架构的分子机制仍知之甚少。本研究通过在完整活体心脏中应用邻近蛋白质组学（proximity proteomics）技术，鉴定了二联体周边富集的蛋白质。