RNA-Seq of circHIPK3 knockdown (KD) samples in UMUC3 and J82

We profile gene expression upon circHIPK3 KD in two bladder cancer cell lines UMUC3 and J82 Cells were treated with a control scramble (scr) siRNA or an siRNA that specifically knock down the expression of circHIPK3. mRNA profiling upon treatment were generated using QuantSeq

本研究针对两种膀胱癌细胞系UMUC3和J82，开展环状RNA HIPK3（circHIPK3）敲低后的基因表达谱分析。实验中，两组细胞分别采用阴性对照乱序（scramble, scr）小干扰RNA（small interfering RNA, siRNA），或特异性敲低circHIPK3表达的siRNA进行处理。处理后的mRNA表达谱通过QuantSeq技术完成构建。