MATR3 ChIP-seq analysis from C2C12 differentiated myotubes upon pCharme lncRNA knockdown

Identification of pCharme dependent Matrin3 chromatin binding sites by ChIP-seq analysis performed in pCharme depleted vs control myoutubes Overall design: C2C12 cells were transfected wirth GAP-scr vs GAP-1 LNA gapmers against pCharme lncRNA and induced to differentiate. Cells were collected at day2 upon the induction of differentiation, the chromatin was collected and sonicated, and ChIP-Seq was performed.

通过在pCharme敲低组与对照肌管中开展的染色质免疫共沉淀测序（ChIP-seq）分析，鉴定依赖pCharme的Matrin3染色质结合位点。
实验整体设计：将C2C12细胞分别转染靶向pCharme长链非编码RNA（long non-coding RNA, lncRNA）的GAP-1锁核酸gapmer（LNA gapmer）与GAP-scr对照锁核酸gapmer，诱导细胞分化后于分化诱导第2天收集细胞，提取染色质并进行超声破碎，随后开展ChIP-seq实验。