Replication Data for: Innervated adrenomedullary microphysiological system to model prenatal nicotine and opioid exposure

To investigate mechanisms regulating adrenomedullary innervation, we developed compartmentalized 3D microphysiological systems (MPS) by exploiting the meniscus pinning effect via GelPins, capillary pressure barriers between cell-laden hydrogels. The MPS comprises discrete 3D cultures of adrenal chromaffin cells and preganglionic sympathetic neurons within a contiguous bioengineered microtissue. Using this model, we demonstrate that adrenal chromaffin innervation plays a critical role in hypoxia-medicated catecholamine release. Furthermore, opioids and nicotine were shown to affect adrenal chromaffin cell response to a reduced oxygen environment, but neurogenic control mechanisms remained intact. GelPin containing MPS represent an inexpensive and highly adaptable approach to study innervated organ systems and improve drug screening platforms by providing innervated microenvironments. Cardiomyocyte beat rate is found through video analysis.

为探究调控肾上腺髓质神经支配的机制，本研究借助GelPins（细胞负载水凝胶间的毛细管压力屏障）实现的弯液面钉扎效应，构建了分隔式3D微生理系统（MPS）。该MPS由连续生物工程微组织内的肾上腺嗜铬细胞与节前交感神经元的独立3D培养体系构成。利用该模型，我们证实肾上腺嗜铬细胞的神经支配在缺氧介导的儿茶酚胺释放过程中发挥关键作用。此外，研究表明阿片类物质与尼古丁可影响肾上腺嗜铬细胞对低氧环境的应答，但神经源性调控机制仍维持完整。搭载GelPins的MPS提供了一种低成本且高度可适配的研究受神经支配器官系统的方案，并可通过构建神经支配微环境优化药物筛选平台。本研究通过视频分析获取心肌细胞搏动频率。