Identification of cranial-specific neural crest genes by comparative transcriptomics. Gallus gallus

We employ RNA-seq of FACS sorted cell populations to identify genes that are enriched in cranial neural crest in relationship to the trunk. Overall design: Transcriptional profiling of delaminating cranial and trunk neural crest subpopulations.

我们借助荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分离得到细胞群体，开展RNA测序（RNA-seq）实验，旨在鉴定相较于躯干神经嵴，在颅神经嵴中特异性富集的基因。实验整体设计：对处于迁出阶段的颅部与躯干神经嵴亚群进行转录谱分析。