PARP1 binds and regulates expression of extracellular matrix genes in renal tubular epithelial cells [RIP-Seq]

To exploit the potential function of PARP1 during renal IRI by binding target RNA to regulate its expression, we obtained PARP1 bound peaks in proximal tubular epithelial cell (HK-2) by iRIP-seq for the first time, which showed that the bind peaks of PARP1_IP group is more enriched at GGUAA-rich motifs and PARP1 preferentially bound to the downstream of TSS (transcription start site) and upstream of TTS (transcription termination site). iRIP-seq analysis was used to decipher RBP binding sites and revealed the RNA that interacts with PARP1 within the genome of HK-2 cells. RIP-seq analysis of PARP1 IP versus input in HK-2 cells

为探究多聚ADP核糖聚合酶1（PARP1）在肾缺血再灌注损伤（renal IRI）中的潜在功能——即通过结合靶RNA调控其表达——我们首次在近端肾小管上皮细胞（HK-2）中通过改进型RNA免疫沉淀测序（iRIP-seq）获取了PARP1的结合峰区域。分析结果显示，PARP1免疫沉淀组（PARP1_IP）的结合峰在富含GGUAA的基序处富集更为显著，且PARP1优先结合于转录起始位点（transcription start site, TSS）下游与转录终止位点（transcription termination site, TTS）上游区域。本研究通过iRIP-seq分析解析了RNA结合蛋白（RNA-binding protein, RBP）的结合位点，并揭示了HK-2细胞基因组中与PARP1相互作用的RNA。本次分析针对HK-2细胞中PARP1免疫沉淀组与输入对照组的RIP-seq数据展开。