High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding

This spreadsheet provides the sequences counts for the DNA groups found in the scats of black-browed albatross at New Island and Steeple Jason Island, Falkland Islands; Diego Ramírez and Albatross Islet, Chile; Bird Island, South Georgia; Canyon des Sourcils Noirs, Kerguelen Archipelago, France; Macquarie Island, Australia; and Campbell albatross at Campbell Island, NZ.

Scat samples were collected in 2013/14 and 2014/15 at New Island, Steeple Jason Island, Macquarie Island, Campbell Island and Bird Island; in 2013/14 and 2015/16 at Kerguelen; in 2014/15 and 2015/16 at Albatross Islet and in 2013/14 at Diego Ramírez. Samples were collected during Incubation (Oct-Nov), early chick-rearing (Dec-Jan) or late-chick rearing (Feb-Mar). Due to the availability of birds at the colony, samples were predominantly collected from adults during incubation and early chick-rearing and chicks during late chick rearing. Samples sizes were too low during this study to directly compare dietary differences between chicks and adults; however, dietary comparisons between breeding stages were examined for sites where samples were collected during multiple breeding stages.

Samples were PCR amplified with a universal metazoan primer set that is highly conserved and amplifies a region of the nuclear small subunit ribosomal DNA gene (18S rDNA). Details of the molecular methods and synthesis of this data can be found in: McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N. High occurrence of jellyfish predation by black-browed and Campbell albatross identified by DNA metabarcoding. Molecular Ecology.

本电子表格提供以下地点黑眉信天翁粪便中DNA组的序列计数：福克兰群岛的新岛与尖顶杰森岛、智利的迭戈·拉米雷斯岛与信天翁小岛、南乔治亚的鸟岛、法国凯尔盖朗群岛的黑眉峡谷、澳大利亚的麦夸里岛；以及新西兰坎贝尔岛坎贝尔信天翁粪便中的DNA组序列计数。

粪便样本的采集时间与地点如下：2013/14及2014/15年于新岛、尖顶杰森岛、麦夸里岛、坎贝尔岛与鸟岛；2013/14及2015/16年于凯尔盖朗群岛；2014/15及2015/16年于信天翁小岛；2013/14年于迭戈·拉米雷斯岛。样本采集时段涵盖孵化期（10-11月）、雏鸟早期抚育期（12-1月）及雏鸟后期抚育期（2-3月）。受繁殖群鸟类可获得性的限制，样本在孵化期与雏鸟早期抚育期主要采自成鸟，而在雏鸟后期抚育期则采自雏鸟。本研究样本量较小，无法直接比较雏鸟与成鸟的饮食差异；但对于存在多繁殖阶段样本的地点，已开展不同繁殖阶段间的饮食差异分析。

样本通过高度保守的通用后生动物引物组进行PCR扩增，该引物组可靶向扩增核小亚基核糖体DNA基因（18S rDNA）的特定区域。分子实验方法及数据合成的详细信息，请参见McInnes, J.C., Alderman, R., Raymond, B., Lea, M-A., Deagle, B., Catry, P., Gras, M., Phillip, R.A., Stanworth, A., Suazo, C., Thompson, D., Weimerskirch, H., Gras. M., and Jarman, S.N.发表于《Molecular Ecology》的论文《通过DNA宏条形码（DNA metabarcoding）揭示黑眉信天翁与坎贝尔信天翁对水母的高捕食率》。