The microRNA bantam regulates a developmental transition in epithelial cells that restricts sensory dendrite growth

Microarray profiling of GFP-marked epithelial cells isolated by FACS from 1st and 3rd instar wild type and mutant Drosophila larvae. Multiple independent replicates collected for each population/timepoint combination. RNA isolated and amplified from FACS-sorted populations. Hybridized as two-color experiment against a common pooled reference.

本数据集针对从1龄与3龄野生型及突变型果蝇幼虫中，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分离得到的绿色荧光蛋白（Green Fluorescent Protein，GFP）标记上皮细胞开展微阵列芯片分析。针对每一组群体/时间点组合，均收集了多组独立生物学重复样本。从经FACS分选的细胞群体中分离RNA并完成扩增。以双色杂交实验方案与统一混合参考样本进行杂交。