c8be300c-3791-4ebe-b44a-202025b9d721 - samples

PopCol is a cohort study in Stockholm, Sweden that includes a data-rich set of individuals with data available from bowel symptoms questionnaires, gastroenterology visits and biospecimens (genotype and 16S sequencing from blood and stool samples, respectively). Genotyping was carried out using the Illumina HumanOmniExpressExome-8v1 arrays at the SciLifeLab NGI facility in Uppsala, Sweden. Fecal DNA was extracted from samples kept at -80°C using Qiagen 5 QIAamp DNA Stool Mini Kits and analyzed using 16S rRNA gene amplicon sequencing (in the V1-V2 hypervariable region). This was performed on the Illumina MiSeq platform at the Institute of Clinical Molecular Biology (IKMB) in Kiel, Germany. Of these, six PopCol participants were PPI users and 12 used antibiotics. The study was approved by the local Committee of Research Ethics (Forskningskommitté Syd) at Karolinska Institutet, Stockholm, in November 2001. Written informed consent was obtained from all participantsEGA dataset EGAD00001007071

PopCol是一项在瑞典斯德哥尔摩开展的队列研究，纳入了一批数据详实的个体受试者，相关数据涵盖肠道症状调查问卷、胃肠病学就诊记录以及生物样本检测数据（分别来自血液样本的基因型检测与粪便样本的16S测序数据）。基因分型检测采用Illumina HumanOmniExpressExome-8v1芯片，在瑞典乌普萨拉的SciLifeLab NGI设施完成。粪便DNA从保存于-80℃的样本中提取，使用Qiagen 5 QIAamp DNA粪便微量试剂盒，并针对16S rRNA基因V1-V2高变区开展扩增子测序分析。该测序工作在德国基尔临床分子生物学研究所（IKMB）的Illumina MiSeq平台上完成。其中，6名PopCol受试者为质子泵抑制剂（Proton Pump Inhibitor, PPI）使用者，另有12名受试者曾使用抗生素。该研究于2001年11月经瑞典斯德哥尔摩卡罗林斯卡学院（Karolinska Institutet）当地研究伦理委员会Forskningskommitté Syd审批通过。所有受试者均已签署书面知情同意书。本研究数据隶属于欧洲基因组-表型组数据库（European Genome-phenome Archive, EGA），数据集编号为EGAD00001007071。