RNA SEQ data of mouse CD8+ Teff treated with LA/LC
收藏科学数据银行2022-10-17 更新2026-04-23 收录
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CD8+ T cells derived from mouse spleen were activated by CD3/28 in vitro and divided into the following four groups. 1. The control group was treated with solvent (water). 2. Lactic acid treatment group was treated with 10mM lactic acid for 3 hours; 3. Lithium carbonate group was treated with 1mM lithium carbonate for 3 hours; 4. The lactate/lithium carbonate combined treatment group was treated with 1mM lithium carbonate combined with 10mM lactate for 3 hours. The samples were lysed with Trizol and then total RNA was extracted for RNASEQ sequencing. Sample TE11-13 was CTR2 in the control group; Sample LA11-13 was LAC2 in lactate treatment group. LC11-13 was LCcase2 in lithium carbonate treatment group. Sample LALC11-13 was LALCcase2 in the lactate/lithium carbonate combined treatment group
从小鼠脾脏分离的CD8+ T细胞（CD8+ T cells）经体外CD3/28激活后，被划分为以下四组：1. 对照组：采用溶剂（水）处理；2. 乳酸处理组：以10mM乳酸处理3小时；3. 碳酸锂处理组：以1mM碳酸锂处理3小时；4. 乳酸/碳酸锂联合处理组：以1mM碳酸锂联合10mM乳酸处理3小时。随后使用Trizol裂解所有样本，提取总RNA并进行RNASEQ测序。其中，样本TE11-13对应对照组的CTR2；样本LA11-13对应乳酸处理组的LAC2；样本LC11-13对应碳酸锂处理组的LCcase2；样本LALC11-13对应乳酸/碳酸锂联合处理组的LALCcase2
提供机构：
JING WEI MA; Huazhong University of Science and Technology; YAO YAO TAN; BO HUANG
创建时间：
2022-10-10
