X-ray Velocimetry, CT, Flexivent and Histology dataset of mice with lung tumours.

Data supporting the paper 'Mapping Lung Cancer Ventilation Dynamics: A Pilot Mouse Study Using Functional Imaging and Lung Mechanics'

Abstract:
In vivo models that replicate and reproduce human lung cancer and its response to therapy are necessary for the development of new therapeutic strategies and understanding drug resistance. Imaging lung tumors in live animals to monitor tumor growth and response to therapy is challenging due to the location of the lungs and their constant movement during breathing. Additionally, methods such as computed tomography (CT) only provide structural information and not functional information about how well the lungs are working.
X-ray velocimetry (XV) is a novel functional lung imaging technique that generates 3D maps of regional lung expansion during breathing. In other lung diseases it has been shown to provide spatial information on where ventilation changes occur. The aim of this pilot study was to use XV and flexiVent lung mechanics assessments to determine the effect of tumor growth on lung function in mice at 2- or 3-weeks post tumor induction, and to evaluate the efficacy of these two tools.
Histological analysis showed that tumour growth was not uniform between animals. At 3-weeks post tumor induction, some XV ventilation and flexiVent lung mechanics parameters were significantly different from baseline metrics. In addition, the forced expiratory volume, small-scale ventilation heterogeneity, and the average CT gray value correlated with the tumour counts from the histology. In some mice XV revealed localised regions with altered expansion rates.
This pilot study demonstrated that changes in lung function can be identified following tumor induction, and that the model and techniques could be used in the future to determine response to anti-tumor drugs.

本数据集支撑论文《肺癌通气动力学图谱：一项基于功能成像与肺力学检测的小鼠预实验》（Mapping Lung Cancer Ventilation Dynamics: A Pilot Mouse Study Using Functional Imaging and Lung Mechanics）。

摘要：
复制人类肺癌及其治疗响应的体内模型，是开发新型治疗策略、解析药物耐药性的必要工具。由于肺部的解剖位置与呼吸过程中的持续运动，在活体动物中成像以监测肺肿瘤生长及治疗响应极具挑战。此外，计算机断层扫描（computed tomography, CT）等成像方法仅能提供结构信息，无法反映肺部工作状态的功能信息。
X射线测速法（X-ray velocimetry, XV）是一种新型功能肺成像技术，可生成呼吸过程中肺部区域扩张的三维图谱。在其他肺部疾病研究中，该技术已被证实能够提供通气改变发生位置的空间信息。本预实验旨在利用X射线测速法（XV）与flexiVent肺力学检测系统，明确肿瘤诱导后2周或3周时，肿瘤生长对小鼠肺功能的影响，并评估这两种工具的应用效能。
组织学分析显示，不同个体间的肿瘤生长并不均匀。在肿瘤诱导后3周时，部分XV通气参数与flexiVent肺力学参数与基线指标存在显著差异。此外，用力呼气容积、小尺度通气异质性以及CT平均灰度值，与组织学检测的肿瘤计数呈显著相关。在部分小鼠中，XV成像揭示了局部区域的扩张速率发生改变。
本预实验证实，肿瘤诱导后可检测到肺功能改变，且该小鼠模型与上述检测技术未来可用于评估抗肿瘤药物的响应效果。