TMT Multiplexing-Based Quantitative Analysis of Pathogenic Th2 Proteomes.

To investigate the regulation of pathogenic Th2 (pTh2) cells and the role of histone deacetylase 1 (HDAC1) in the respective processes, we used a new in vitro protocol for generating pTh2 cells and performed qunatitative mass spectrometry analysis based on TMTpro 16plex multiplexing to analyze proteomes. We profiled classical Th2 and pTh2 cells from wild type (WT) mice and mice with a T cell-specific deletion of HDAC1 (HDAC1-cKO).

为探究致病性Th2细胞（pathogenic Th2 cells，简称pTh2）的调控机制以及组蛋白去乙酰化酶1（histone deacetylase 1，HDAC1）在对应过程中的作用，本研究采用全新体外实验方案构建pTh2细胞，并基于TMTpro 16重标记多重定量技术开展定量质谱分析以解析蛋白质组。我们对野生型小鼠（wild type，简称WT）以及T细胞特异性敲除HDAC1（HDAC1-cKO）小鼠来源的经典Th2细胞与pTh2细胞进行了蛋白质组谱分析。