TonEBP/NFAT5 controls inflammatory transcriptional response to TNF-a in nucleus pulposus cells

RNA sequencing of nucleus pulposus cells transduced with shRNA (control or TonEBP-targeted) and either untreated or treated with TNF-a (24h) Overall design: Total mRNA was collected from primary nucleus pulposus cells and subjected to RNA sequencing, n=3 for all experimental groups

本数据集针对转导短发夹RNA（shRNA，分为对照组与TonEBP靶向组）且分别经肿瘤坏死因子-α（TNF-α）处理24小时或未经处理的髓核细胞开展RNA测序。实验设计概况：从原代髓核细胞中提取总mRNA并进行RNA测序，所有实验组的重复样本数均为n=3。