Glycolytic shift during West Nile virus infection provides new therapeutic opportunities [2DG and DGA compounds]. Glycolytic shift during West Nile virus infection provides new therapeutic opportunities [2DG and DGA compounds]

To investigate the in vivo metabolic changes during WNV infection in a mouse model, we infected mice with WNV NY99 to compare to uninfected (mock) mice. We also use two compounds targeting energetic metabolism to analyze their effects on WNV infection We then performed gene expression profiling analysis using data obtained from RNA-seq from infected vs uninfected mice and treated mice. Overall design: Comparative gene expression profiling analysis of brain RNA-seq data for mice infected with WNV vs uninfected mice

为探究西尼罗病毒（WNV）感染小鼠模型体内的代谢变化，我们使用西尼罗病毒NY99毒株感染小鼠，并与未感染（模拟感染，mock）小鼠进行对照。此外，我们选用两种靶向能量代谢的化合物，分析其对西尼罗病毒感染的影响。随后，我们利用感染组、未感染组以及给药处理组小鼠的RNA-seq测序数据，开展基因表达谱分析。整体实验设计：对比分析西尼罗病毒感染小鼠与未感染小鼠的脑组织RNA-seq基因表达谱数据。