Transcriptome analysis of total RNA from Rhodobacter sphaeroides 2.4.1 wildtype and StsR (RSs0827) deletion strain during different growth phases

The sRNA StsR (formerly RSs0827) was first described as an sRNA induced upon iron starvation and was later demonstrated to be the most highly induced RNA in late stationary phase in the phototrophic Alphaproteobacterium R. sphaeroides. StsR is under control of RpoHI/RpoHII and therefore induced by multiple stress factors. To investigate the function of this sRNA on the hole transcriptome of R. sphaeroides we performed RNAseq with cultures of the exponential growth (6 h) phase, stationary growth phase (72 h / 144 h) and outgrowth phase. Cultures were grown under microaerobic growth conditions in biological triplicates. Overall design: This data includes the transcriptome data of two different strains each in triplicates: Rhodobacter sphaeroides 2.4.1 (wildtype) and R. sphaeroides 2.4.1 StsR deletion strain (d0827). Total RNA of all strains was isolated and sequenced.

小RNA（small RNA，sRNA）StsR（原名为RSs0827）最初被报道为一种在铁饥饿条件下诱导表达的sRNA，后续研究证实其在光合α-变形菌（Alphaproteobacterium）球形红杆菌（Rhodobacter sphaeroides，R. sphaeroides）的稳定期晚期为表达量最高的诱导型RNA。StsR受RpoHI/RpoHII调控，因此可被多种胁迫因子诱导表达。为探究该sRNA对球形红杆菌全转录组的调控功能，本研究针对指数生长期（培养6小时）、稳定生长期（培养72小时/144小时）以及复苏生长期的培养物开展了RNA测序（RNA-seq）。所有培养物均在微好氧生长条件下培养，并设置三次生物学重复。整体实验设计：本数据集包含两种菌株的转录组数据，每种均设置三次生物学重复：球形红杆菌2.4.1野生型菌株，以及球形红杆菌2.4.1 StsR缺失菌株（Δ0827，原标注为d0827）。研究人员对所有菌株的总RNA进行提取及测序。