Effect of WY-14643 on Rat Primary Hepatocytes.. Rattus norvegicus

This study provides an evaluation of changes in gene expression associated with WY-14643 treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses 8 uM and 200 uM) of WY-14643 and 1% DMSO vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis. Overall design: This series is part of a SuperSeries in which primary rat hepatocytes were treated with two doses of ten chemical compounds (and corresponding vehicle controls) for 24 and 48 hours. Each compound/vehicle treatment group was an individual study performed at different times. Each study was analyzed separately and themes common between studies were reported. Time Course/Dose Response

本研究针对体外培养大鼠肝细胞经WY-14643处理后相关基因表达的变化展开评估。实验采用8 μM、200 μM两种剂量的WY-14643以及1%二甲基亚砜（DMSO）溶剂对照，对原代大鼠肝细胞分别处理24小时与48小时，每个处理组均设置5次生物学重复。随后对细胞进行提取，并对RNA进行基因芯片（microarray）分析。
整体实验设计：本系列隶属于某一超级数据集（SuperSeries），该超级数据集针对10种化学化合物及其对应溶剂对照，以两种剂量分别处理原代大鼠肝细胞，处理时长为24小时与48小时。每个化合物/溶剂对照处理组为一项独立研究，于不同时间开展。各项研究均单独进行分析，并对不同研究间共通的研究主题进行报道。本实验涵盖时间进程与剂量响应两类设计。