Proteomic analyisis of cultured human podocytes stimulated with TNFa - cell pellet

TNFa stimulated human cultured podocytes compared with unstimulated (vehicle control (VC)) podocytes. Incubation of podocytes with high concentrations of TNF alpha led to an overexpression of ICAM1 in particular. We studied this system as a comparator for organoids also stimulated with the same concentration of TNFa. Four conditions (6 replicates each) 1) treatment (5 ng/mL TNFa) for 24 hours 2) control (PBS) for 24h 3) treatment (5 ng/mL TNFa) for 48 hours 4) control (PBS) for 48h

本数据集对比了经肿瘤坏死因子α（TNFα）刺激的人培养足细胞与未经刺激的溶剂对照（VC）组足细胞。研究观察到，以高浓度TNFα孵育足细胞时，可特异性诱导细胞间黏附分子1（ICAM1）的过表达。本研究将该细胞培养体系作为对照，用于比对经相同浓度TNFα刺激的类器官。本数据集包含4组实验条件，每组设置6次生物学重复：1）5 ng/mL TNFα处理24小时；2）磷酸盐缓冲液（PBS）对照处理24小时；3）5 ng/mL TNFα处理48小时；4）PBS对照处理48小时。