Wingless-2.331 300kb candidate region targeted capture array. Gallus gallus strain:Wingless-2.331 | breed:Single comb white leghorn

The candidate causative region, retaining the mutation responsible for the wingless-2 mutant phenotype, resides within a 227 kb region of chicken chromosome 12. 300 kb (227 kb and flanking DNA) is sequenced in a cohort of individuals from the congenic inbred line Wingless-2.331 to obtain deep coverage in each bird across this region. Comparisons between individuals are made in an effort to distinguish the potentially causative variation, which ultimately may be used to enhance the use of this line as a developmental model for comparable human disorders.

保留了导致翅膀缺失-2（wingless-2）突变表型的突变的候选致病区域，坐落于鸡12号染色体的227 kb区段内。针对同系近交系Wingless-2.331的个体队列，对涵盖该227 kb区段及其侧翼DNA的300 kb区域进行测序，以实现该区域在每只受试个体中的高深度测序覆盖。通过比对不同个体的序列数据，旨在甄别潜在的致病变异；该变异最终可用于提升该品系作为对应人类发育疾病模型的应用价值。