Knockdown of Activin B stabilizes actin stress fibers in ccRCC cells.

(A) Subconfluent parental 786.0 and RCC10 cells, control clones (786.0 neo#1, RCC10 scr#3) and Activin B knockdown clones (786.0 si1-βB#2, RCC10 si2-βB#4), respectively, were maintained in the presence of 10% FCS or serum starved (0% FCS) for 3 hours. Micrographs show TRITC-labeled Phalloidin staining of the actin cytoskeleton. (B) and (C) Quantification of the indicated 786.0 (B) and RCC10 cells (C) with actin stress fibers upon serum starvation for 3 hours. Phalloidin stained cells were classified by microscopic analysis and at least 200 cells were counted per experiment. Bars represent the mean of four independent experiments, error bars indicate standard deviation. Statistical significance was determined by ANOVA analysis and denoted by asterisks: ***P<0.001.

(A) 亚汇合状态的亲本786.0细胞与RCC10细胞、对照克隆株（786.0 neo#1、RCC10 scr#3）及激活素B敲低克隆株（786.0 si1-βB#2、RCC10 si2-βB#4），分别在含10%胎牛血清（FCS）的培养基中培养，或施以3小时血清饥饿处理（培养基血清浓度为0%，即0% FCS）。显微照片展示了肌动蛋白细胞骨架的四甲基异硫氰酸罗丹明（TRITC）标记鬼笔环肽染色结果。(B) 和 (C) 分别为经3小时血清饥饿处理的786.0细胞(B)及RCC10细胞(C)中，携带肌动蛋白应力纤维的细胞占比的量化分析结果。通过显微观察对鬼笔环肽染色的细胞进行分类，每次实验至少计数200个细胞。柱状图代表四次独立实验的平均值，误差棒表示标准偏差。统计学显著性通过方差分析（ANOVA）计算得出，以星号标注显著性水平：***P<0.001。