STIL/AURKA axis promotes cell proliferation by influencing primary cilia formation in bladder cancer

Our research showed that primary cilia was reduced in human bladder cancer (BLCA) cells, and PC deficiency promote cell proliferation. However, the concrete mechanisms remain unknown. SCL/TAL1 interrupting locus (STIL), a PC-related protein, was screened in our previous study and could influence the cell cycle by regulating PC in tumor cells. To elucidate the function of STIL for PC to explore the underlying mechanism of PC in BLCA, we establisded siNC and siSTIL EJ cells. Overall design: Comparative gene expression profiling analysis of RNA-seq data for EJ-siNC and EJ-siSTIL cells.

本研究发现，人类膀胱癌（BLCA）细胞中的初级纤毛（primary cilia，以下简称PC）数量减少，且PC缺陷可促进细胞增殖。然而其具体分子机制目前仍不明确。SCL/TAL1打断位点（SCL/TAL1 interrupting locus，STIL）作为一种PC相关蛋白，本团队在前期研究中完成筛选，其可通过调控肿瘤细胞内的PC影响细胞周期。为阐明STIL在PC调控中的功能，进而探究PC在BLCA发生发展中的潜在机制，本研究构建了转染阴性对照小干扰RNA（siNC）及靶向STIL的小干扰RNA（siSTIL）的EJ细胞系。实验整体设计：对EJ-siNC与EJ-siSTIL细胞的RNA测序（RNA-seq）数据进行比较基因表达谱分析。