ANTROPOMETRIC INDECES AS POTENTIAL PREDICTORS OF CARDIOMETABOLIC RISK FACTORS IN COLLEGE STUDENTS.

At the PesqClin laboratory – Hospital Universitário Onofre Lopes/ UFRN, the appropriate structure was set up to carry out all the steps necessary for data collection, according to the procedures and instruments: application of anamnesis on the history of personal and family diseases, the body mass was measured using a Sanny® digital scale according to the standardization described by Gordon (GORDON, C. C.; CHUMLEA, W. C.; ROCHE, A. F. Stature recumbert, lenght, and weight. In: LOHMAN, T. G.;ROCHE, A. F., 1988), height was measured using a Sanny® stadiometer as standardized by Gordon(GORDON, C. C.; CHUMLEA, W. C.; ROCHE, A. F. Stature recumbert, lenght, and weight. In: LOHMAN, T. G.;ROCHE, A. F., 1988), the abdominal/waist circumference was measured following the procedure described by Costa (COSTA, 2001), using Sanny® anthropometric metal tape. BMI was calculated by dividing mass in kilograms by the square of height in meters. The ABSI was calculated according to the equation proposed by Krakauer and Krakauer (Krakauer &amp; Krakauer, 2012). The BRI was calculated following the equation proposed by Thomas et al (Thomas et al., 2013) BRI=364.2−365.5× 1−(WC/(2π))2/(0.5Height)2. WHtR was determined by dividing waist circumference (cm) by height (cm)(Pitanga &amp; Lessa, 2006). <pre><code> Blood pressure was measured with a semiautomatic device (OMRON – HEM 705 CP) following the criteria of the VI Brazilian Guidelines on Arterial Hypertension (2010), the final result being considered the average of three measurements performed with an interval of two minutes between each one.</code></pre> <pre><code> To standardize the biochemical evaluation, 10 ml blood samples were collected by puncture of the antecubital vein, always performed by the same professional, always between 7:00 and 8:00 am, after fasting for 12 hours. After peripheral blood collection, serum concentrations of fasting glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol and triglycerides were determined.</code></pre> <pre><code> All dosages mentioned above were performed by enzymatic-colorimetric assays for "in vitro" diagnoses, specific for each dosage, considering the cut-off values according to the NCEP-ATP III diagnostic criteria (Expert Panel on Detection, Evaluation, 2001) for metabolic syndrome, triglycerides ≥150mg/dl, HDL (male) &lt; 40mg/dl and HDL (female) &lt; 50mg/dl, fasting glucose ≥100mg/dl.</code></pre> <pre><code> For the analysis of dosages, commercial kits Labtest Diagnóstica-SA® were used, in the Labmax Plenno equipment (Labtest®, Lagoa Santa/MG) and for the determination of LDL-cholesterol levels, the Martin formula was used (Martin et al., 2013) (total cholesterol – HDL-cholesterol - [triglycerides/factor]) (factor/;3.1 to 11.9). </code></pre>

本研究的数据采集工作于佩斯克林实验室（PesqClin）——奥诺弗雷·洛佩斯大学医院/巴西联邦共和国里约格兰德联邦大学（UFRN）内开展，搭建了符合规范的完整实验架构以完成所有数据采集步骤，严格遵循既定流程与工具执行操作：通过问诊（anamnesis）收集个人与家族疾病史；采用Sanny®数字体重秤，依据Gordon等人的标准化方案（GORDON, C. C.; CHUMLEA, W. C.; ROCHE, A. F. Stature recumbert, lenght, and weight. In: LOHMAN, T. G.;ROCHE, A. F., 1988）测量体重；使用Sanny®身高计，同样遵循Gordon等人的上述标准化方案测量身高；采用Sanny®人体测量金属软尺，按照Costa（COSTA, 2001）描述的操作流程测量腹围/腰围。本研究通过体重（千克）除以身高（米）的平方计算身体质量指数（BMI）。身体体型指数（ABSI）依据Krakauer与Krakauer提出的公式（Krakauer & Krakauer, 2012）计算得到。身体圆润指数（BRI）则遵循Thomas等人（Thomas et al., 2013）提出的公式：BRI=364.2−365.5×√[1−(WC/(2π))²/(0.5Height)²]。腰围身高比（WHtR）通过腰围（厘米）除以身高（厘米）计算得出（Pitanga & Lessa, 2006）。<pre><code> 血压测量采用半自动设备（OMRON – HEM 705 CP，欧姆龙），严格遵循《第六届巴西动脉高血压临床指南（2010）》的标准执行，每次测量间隔2分钟，共完成3次测量，取三次测量的平均值作为最终血压结果。</code></pre><pre><code> 为标准化生化检测流程，所有血液样本均通过肘前静脉穿刺采集，每次采集均由同一名专业人员操作，采集时间固定为每日早上7:00至8:00，且受试者需提前12小时禁食。外周血采集完成后，检测血清中空腹血糖、总胆固醇、高密度脂蛋白胆固醇（HDL-cholesterol）、低密度脂蛋白胆固醇（LDL-cholesterol）以及甘油三酯的浓度。</code></pre><pre><code> 上述所有检测项目均采用适配体外诊断的酶比色法，针对不同检测项目选用专属检测试剂，检测截断值依据美国国家胆固醇教育计划成人治疗组第三次指南（NCEP-ATP III，Expert Panel on Detection, Evaluation, 2001）中代谢综合征的诊断标准设定：甘油三酯≥150mg/dl，男性高密度脂蛋白胆固醇＜40mg/dl、女性高密度脂蛋白胆固醇＜50mg/dl，空腹血糖≥100mg/dl。</code></pre><pre><code> 本研究检测过程中使用莱测诊断公司（Labtest Diagnóstica-SA®）的商业化试剂盒，检测设备为Labmax Plenno（Labtest®, 拉戈阿圣塔/米纳斯吉拉斯州）；低密度脂蛋白胆固醇水平则采用Martin等人（Martin et al., 2013）提出的Martin公式计算：LDL-cholesterol=总胆固醇−HDL-cholesterol−[甘油三酯/校正因子]，其中校正因子取值范围为3.1至11.9。</code></pre>