MOBHunter_Table1Files_Performance

MOBHunter, a pipeline combining 10 high-performing tools and custom algorithms to detect unique genomic regions, diagnostic markers and genetic boundaries of MGE. MOBHunter integrates these predictions, offers evidence-based classifications, and produces GenBank-compatible outputs for visualization, creating a comprehensive platform for MGE analysis of complete genomes of bacteria and archaeas.The process starts with the pipeline setting the environment in which it will work. The script checks for the availability of .gbk files in the defined path. Then, it will proceed to convert the .gbk files into formats required by the other programs. Converted formats include .faa, .ffn, .fna and .ptt.After the required files are created, the pipeline will run all the incorporated 10 programs. The outputs are then processed in a consensus, and the consensus will conduct the scoring of each hit. If a hit is classified by all the incorporated programs, it will receive a higher score.

MOBHunter是一款集成10款高性能工具与自定义算法的分析流程，可用于检测可移动遗传元件（MGE，Mobile Genetic Elements）的独特基因组区域、诊断标记及遗传边界。该流程整合上述预测结果，提供循证分类方案，并生成兼容GenBank格式的可视化输出文件，从而构建出一套可用于细菌与古菌完整基因组MGE分析的综合性平台。分析流程的运行始于环境配置步骤，脚本首先会检查指定路径下是否存在.gbk格式文件，随后将.gbk格式文件转换为其他分析工具所需的格式，转换后的格式包括.faa、.ffn、.fna以及.ptt。所需文件生成完成后，流程将运行全部集成的10款工具，随后对所有工具的输出结果开展共识整合分析，并对每一条比对命中结果进行打分。若某条比对命中结果被所有集成工具共同判定分类，则将获得更高的评分。