HDACs Immunoreactivities in Monoaminergic and Neuropeptidergic Neurons.

The percentage of immunoreactive cells was assessed at the confocal laser scanning microscopy. Values are means ± S.E.M. Nuclear or cytoplasmic localization of HDACs was determined based on the colocalization with Hoechst 33342. The extent of HDACs immunoreactivities was determined on the intensity and area of the immunoreactivities: “+++”, strong immunoreactivity; “++”, moderate immunoreactivity; “+”, weak immunoreactivity; “−“, no immunoreactivity above background.

采用激光共聚焦扫描显微镜（Confocal Laser Scanning Microscopy）对免疫反应阳性细胞的百分比进行评估。数值以平均值±标准误（S.E.M.）表示。组蛋白去乙酰化酶（HDACs）的细胞核或细胞质定位，通过其与赫斯特33342（Hoechst 33342）的共定位情况进行判定。组蛋白去乙酰化酶的免疫反应活性程度，依据免疫反应的信号强度与面积进行分级："+++"代表强免疫反应；"++"为中等强度免疫反应；"+"为弱免疫反应；"−"表示无高于背景的免疫反应。