Xanthomonas citri pv. citri strain:03-1638-1-1 Genome sequencing. Xanthomonas citri pv. citri strain:03-1638-1-1

Xanthomonas citri, the causal agent of citrus canker, represents a well-studied pathosystem due to the detailed understanding of pathogen biology using a combination of approaches including mutational analysis and, more recently, by sequencing of hundreds of strains of this pathogen. Among various pathogenicity factors deployed by X. citri during disease establishment, the most important and widely studied factor includes a type III effector family of Transcriptional Activator-Like effectors (TALEs). We utilized the power of long reads obtained by PacBio sequencing to enable assembly of a complete genome sequence of strain Xc03-1638-1-1. This study emphasizes the importance of long-read sequencing not only in obtaining intact sequences of TALEs and plasmids, but also identifying rearrangement events including plasmid reshuffling.

Xanthomonas citri（柑橘黄单胞菌）是引发柑橘溃疡病的病原菌，其相关病原物-寄主互作体系已得到广泛且深入的研究——这是因为学界结合突变分析等多种研究手段，以及近年来对该病原菌数百株菌株的测序工作，对其病原生物学已有了详尽的认知。在柑橘黄单胞菌侵染寄主并建立病害的过程中，其所利用的各类致病因子里，研究最为广泛且最为关键的一类为III型效应因子家族中的转录激活因子样效应物（Transcriptional Activator-Like effectors, TALEs）。本研究借助PacBio测序（PacBio sequencing）获得的长读长数据，完成了菌株Xc03-1638-1-1的完整基因组序列组装。本研究凸显了长读长测序技术的重要价值：其不仅能够获取转录激活因子样效应物（TALEs）与质粒的完整序列，还可识别包括质粒重排在内的各类基因组重排事件。