Potential diagnostic utility of recombinant Toxoplasma gondii proteins for detecting anti-Toxoplasma gondii IgG antibodies in horses serum samples

Toxoplasmosis is caused by an intracellular protozoan, Toxoplasma gondii, and is a parasitic disease that occurs in all warm-blooded animals, including humans. Toxoplasmosis is one of the most common parasitic diseases of animals and results in reproductive losses. Diagnosis of toxoplasmosis is currently based on serological assays using native antigens to detect specific anti-T. gondii antibodies. Continuous development of scientific disciplines, such as molecular biology and genetic engineering, allows for the production of new recombinant antigens and provides the basis for new diagnostic tests for the detection of anti-T. gondii antibodies in animal serum samples. In this study, the potential utility of 27 single recombinant Toxoplasma gondii proteins (AMA1, AMA1C, AMA1N, BAG1, BSR4, GRA1, GRA2, GRA2ex2, GRA4, GRA5, GRA5-Trx, GRA6, GRA7, GRA9, LDH1, LDH2, MAG1, MIC1, MIC1ex2, MIC1ex3,4, MIC3, MIC3-Trx, P35, ROP1, SAG1, SAG2, SAG4)  for the detection of anti-Toxoplasma gondii IgG antibodies in horses sera was determined.

弓形虫病（Toxoplasmosis）由刚地弓形虫（Toxoplasma gondii）这种细胞内原生动物引发，是一种可感染包括人类在内所有温血动物的寄生虫病。该病是最常见的动物寄生虫病之一，可造成繁殖损失。目前弓形虫病的诊断依托于使用天然抗原的血清学检测方法，以检测特异性抗刚地弓形虫抗体。随着分子生物学、基因工程等科学学科的持续发展，新型重组抗原的制备成为可能，也为开发用于检测动物血清样本中抗刚地弓形虫抗体的新型诊断试验提供了理论基础。本研究对27种单一重组刚地弓形虫蛋白（AMA1、AMA1C、AMA1N、BAG1、BSR4、GRA1、GRA2、GRA2ex2、GRA4、GRA5、GRA5-Trx、GRA6、GRA7、GRA9、LDH1、LDH2、MAG1、MIC1、MIC1ex2、MIC1ex3,4、MIC3、MIC3-Trx、P35、ROP1、SAG1、SAG2、SAG4）在检测马血清中抗刚地弓形虫IgG抗体方面的潜在应用价值进行了评估。